1. Soaking（petri dish): new or used glassware shall be soaked with clean water to soften and dissolve the attachments. New glassware must be simply brushed with tap water before use, and then soaked in 5% hydrochloric acid overnight; Used glassware is often accompanied by a large amount of protein and oil, which is not easy to brush off after drying, so it should be immersed in clean water immediately after use.
2. Brushing(petri dish): put the soaked glassware into detergent water and brush repeatedly with a soft brush. Do not leave dead corners and prevent damage to the surface finish of utensils. Wash and dry the cleaned glassware for pickling.
3. Pickling(petri dish): pickling is to soak the above utensils into cleaning solution, also known as acid solution, and remove the possible residual substances on the surface of utensils through the strong oxidation of acid solution. Pickling should not be less than six hours, usually overnight or longer. Be careful when placing and taking utensils.
4. Washing: the utensils after brushing and pickling must be fully washed with water. Whether the utensils are washed clean after pickling directly affects the success or failure of cell culture. When manually washing the utensils soaked in acid, each utensil shall be repeatedly "filled with water - emptied" for at least 15 times, and finally soaked with re distilled water for 2-3 times. After drying or drying, it shall be packaged for standby.
