Shanghai Sun-Trine Biotechnology Co., Ltd.

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Pcr Plate in PCR Consumables

2023-04-11
The half-skirt plate has a short edge around the edge of the plate to provide adequate support during pipetting and is used by most PCR instruments.The full skirt PCR plate has an edge panel covering the height of the plate. This plate form is suitable for PCR instruments with protruding modules for safe and stable adaptation. The full skirt also adds mechanical strength, making it ideal for robotic platforms used in automated workflows.
The full plane plate design is suitable for most PCR instruments for easy sealing and handling.The edge raised plate design is optimal for some PCR instruments, helping to balance the heat cap pressure without the need for adapters, ensuring optimal heat transfer and reliable experimental results.
Reaction plates in PCR Consumables are often available in a variety of different color forms to facilitate visual discrimination and identification of samples, especially in high-throughput experiments. Although the color of plastic has no effect on DNA amplification, we recommend using white plastic PCR Consumables or matte treated plastic PCR Consumables to achieve sensitive and accurate fluorescence detection when setting up real-time fluorescent quantitative PCR reactions compared to transparent PCR Consumables.White PCR Consumables improve data sensitivity and consistency by preventing fluorescence refraction from the tubes. When refraction is minimized, more signals are reflected back to the detector, thereby increasing the SNR. In addition, the white tube wall prevents the fluorescence signal from being transmitted to the PCR instrument module, avoiding absorption or inconsistent reflection of the fluorescence signal, thus minimizing the difference in repeated experiments.
The shape of a PCR plate refers to its height, so "low profile" means lower height. The shorter design of low-volume PCR Consumables minimizes the space above the reaction system, reduces the impact of evaporation and improves heat transfer efficiency. Therefore, in the case of simultaneous compatibility, it is recommended to choose low volume PCR Consumables, i.e., "fast" or low plate.
A common feature of PCR plates is a raised edge around the hole. This design may facilitate sealing with a sealing plate membrane to prevent evaporation.

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